Summary auto-generated
Researchers identified and characterized 21 gram-negative bacterial strains isolated from the respiratory tracts of turkeys, chickens, rooks, and a partridge. These isolates were consistently associated with various respiratory infections including tracheitis, pneumonia, and airsacculitis. Through comprehensive taxonomic analysis including DNA-rRNA hybridization, 16S rRNA sequencing, fatty acid analysis, and phenotypic testing, the authors determined these strains constitute a genetically homogeneous new species belonging to rRNA superfamily V. The strains shared greater than 90% DNA-DNA binding values, G+C contents of 37-39 mol%, and were clearly distinct from phenotypically similar organisms such as Riemerella anatipestifer and Capnocytophaga species. The researchers propose the name Ornithobacterium rhinotracheale for this new genus and species, positioning it within the flavobacter-bacteroides phylum but as a distinct lineage separate from other superfamily V members.
Key findings
- A novel gram-negative bacterium, Ornithobacterium rhinotracheale, was identified from 21 avian isolates obtained from respiratory tract infections in poultry and wild birds
- DNA-DNA hybridization values greater than 90% confirmed the strains form a single species with G+C contents between 37-39 mol%
- 16S rRNA sequencing and DNA-rRNA hybridization placed O. rhinotracheale within rRNA superfamily V but as a distinct genus, separate from Flavobacterium and other related taxa
- Fatty acid profiles, protein electrophoresis patterns, and phenotypic characteristics clearly differentiated O. rhinotracheale from phenotypically similar species including Riemerella anatipestifer and Capnocytophaga species
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Abstract
1 Laboratorium voor Microbiologie, Faculteit Wetenschappen, University of Ghent, B-9000 Ghent, Belgium
6 Laboratorium voor Bacteriologie, Faculteit Diergeneeskunde, University of Ghent, B-9000 Ghent, Belgium
2 Klinikum der Philipps-Universität Marburg, Marburg, Germany
3 Provinciaal Verbond voor Dierenziektenbestrijding, Torhout, Belgium
4 Department of Molecular Genetics, Forsyth Dental Center, Boston, Massachusetts 02115
5 Culture Collection, Department of Clinical Bacteriology, University of GÖteborg, S-413 46 GÖteborg, Sweden
7 Royal Veterinary and Agricultural University and National Veterinary Laboratory, Frederiksberg, Denmark
8 Klinik für Geflügel der Tierärztlichen Hochschule, Hannover, Germany