Summary auto-generated
Pseudomonas monteilii is a newly described bacterial species isolated from clinical specimens including bronchial aspirates, urine, bile, stool, and placental tissue. The 10 strains were gram-negative, motile rods that produced fluorescent pigments, catalase, and cytochrome oxidase, but could not liquefy gelatin or grow at 41°C. They utilized numerous organic compounds as carbon sources and possessed multiple esterase and arylamidase enzymes. DNA-DNA hybridization experiments showed that 10 subcluster IIc strains were 86-100% related to each other with minimal DNA divergence, distinguishing them from other Pseudomonas species, including P. putida biovar A. The G+C content averaged 60.5 ± 0.5 mol%, consistent with Pseudomonas sensu stricto. PCR amplification confirmed the presence of the OprI lipoprotein gene characteristic of authentic Pseudomonas species. The type strain CFML 90-60 was isolated from bronchial aspirate. While these organisms were recovered from clinical sources, their clinical significance remains unknown.
Key findings
- P. monteilii forms a homogeneous genomic species with DNA hybridization values of 86-100% among 10 clinical strains, showing ≤1.2°C thermal stability differences
- The species is most closely related to P. putida biovar A (48% hybridization) but shows only 6-54% relatedness to other Pseudomonas species
- P. monteilii possesses multiple defining characteristics including fluorescent pigments, catalase, cytochrome oxidase, arginine dihydrolase, and inability to grow at 41°C
- DNA G+C content of 60.5 ± 0.5 mol% and positive PCR amplification for the OprI lipoprotein gene confirm membership in Pseudomonas sensu stricto
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Abstract
We propose the name Pseudomonas monteilii for a new species of gram- negative, rod-shaped, motile bacteria that were nonhemolytic on blood agar and were isolated from clinical sources. The 10 strains of P. monteilii were incapable of liquefing gelatin. They grew at 10 degrees C but not at 41 degrees C, produced fluorescent pigments, catalase, and cytochrome oxidase, and possessed the arginine dihydrolase system. They were capable of respiratory but not fermentative metabolism. They did not hydrolyze esculin or starch and were able to use benzylamine, alpha- aminobutyrate, D-ribose, L-arabinose, butyrate, valerate, isovalerate, isobutyrate, inositol, phenylacetate, D-alanine, and amylamine. They possessed L-phenylalanine arylamidase, L-lysine arylamidase, L-alanine arylamidase, gamma-glutamyl-transferase, glycyl-phenylalanine arylamidase, L-tryptophan arylamidase, glycyl-L-alanine arylamidase, esterase C4, esterase C6, esterase C8, esterase C9, esterase C10, and esterase C18. DNA relatedness studies revealed that P. monteilii strains formed a homogeneous DNA hybridization group. A total of 57 strains representing previously described or partially characterized taxa belonging to the genus Pseudomonas were 6 to 54% related to P. monteilii. The highest hybridization values were obtained with strains belonging to or related to Pseudomonas putida biovar A. The average G+C content of the DNA was 60.5 +/- 0.5 mol% for four of the P. monteilii strains studied. The type strain of P. monteilii is CFML 90-60 (= CIP 104883); it was isolated from bronchial aspirate and has a G+C content of 60 mol%. The clinical significance of these organisms is not known.