Summary auto-generated
This study reclassifies Alteromonas elyakovii KMM 162T, originally isolated from a Far-Eastern mussel, and five strains isolated from spot-diseased Laminaria japonica fronds as a single species belonging to the genus Pseudoalteromonas. Using DNA-DNA hybridization, phylogenetic analysis of 16S rRNA gene sequences, and phenotypic characterization, the researchers demonstrated that these strains share 71-97% DNA relatedness with each other but show distinctly lower relatedness to other Pseudoalteromonas species (29.9-52.4%). The organisms are polarly flagellated, Gram-negative, marine bacteria with G-C contents of 38.5-38.9 mol%. They produce alginate lyase and laminarinase enzymes and are suspected causative agents of spot disease in kelp. The phenotypic features distinguish the new species primarily through differential utilization of D-mannose, L-tyrosine, and trehalose compared to the closely related Pseudoalteromonas espejiana. The authors propose Pseudoalteromonas elyakovii comb. nov., with type strain KMM 162T (ATCC 700519T), and provide an extended species description including growth characteristics and enzymatic capabilities.
Key findings
- Alteromonas elyakovii KMM 162T and five Laminaria isolates represent a single species with 71-97% DNA-DNA relatedness, supporting reclassification as Pseudoalteromonas elyakovii comb. nov.
- 16S rRNA gene sequence analysis places P. elyakovii within the Pseudoalteromonas genus with 99.8-99.9% similarity to P. distincta, confirming phylogenetic positioning
- The species is distinguished from phenotypically similar P. espejiana by differential utilization of D-mannose, L-tyrosine, and trehalose
- P. elyakovii produces alginate lyase and laminarinase enzymes and is implicated as a causative agent of spot disease in Laminaria japonica crops
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Abstract
A marine bacterium, Alteromonas elyakovii KMM 162(T), which was described recently, and five strains isolated from spot-wounded fronds of Laminaria japonica have been subjected to phylogenetic analysis, and geno- and phenotypic characterization. The phenotypic features of Pseudoalteromonas elyakovii strains were closely related to that of Pseudoalteromonas espejiana IAM 12640(T), but utilization of three carbon compounds (D-mannose, L-tyrosine and trehalose) distinguished both species. The G+C content of Pseudoalteromonas elyakovii was between 38.5 and 38.9 mol%. Pseudoalteromonas elyakovii KMM 162(T) and the five Laminaria isolates constitute a single species different from any other Alteromonas and Pseudoalteromonas species as revealed by DNA--DNA hybridization data, especially Pseudoalteromonas distincta KMM 638(T) (52.4%), Pseudoalteromonas citrea KMM 216 (49.5%), Pseudoalteromonas carrageenovora NCIMB 302(T) (46.9%) and Pseudoalteromonas espejiana IAM 12640(T) (29.9%). All the data indicated that Alteromonas elyakovii KMM 162(T) should be reclassified as Pseudoalteromonas elyakovii and five strains isolated from Laminaria japonica have to be included in the species. Pseudoalteromonas elyakovii comb. nov. (type strain, KMM 162(T)=ATCC 700519(T)) is proposed and a set of phenotypic features which differentiate the Pseudoalteromonas species is described.