Summary auto-generated
Researchers isolated a novel anaerobic bacterium, designated strain Lup 33T, from a wastewater treatment reactor in Mexico. The bacterium is a moderately thermophilic, spore-forming rod that grows optimally at 40°C and pH 7.4. It is heterotrophic, utilizing sugars, peptides, and amino acids as energy sources. Notably, the strain is acetogenic, producing acetate as the primary fatty acid from glucose fermentation, and can facultatively reduce elemental sulfur to sulfide. Based on 16S rRNA gene sequence analysis, the strain belongs to cluster XII of the order Clostridiales, with Clostridium hastiforme as its closest relative (93% similarity). The DNA G+C content is 32.2 mol%. The strain's unique combination of acetogenic metabolism and sulfur-reduction capacity distinguishes it from closely related organisms. On the basis of these phenotypic, genotypic, and phylogenetic characteristics, the authors propose the isolation as a new species in a new genus, Sporanaerobacter acetigenes gen. nov., sp. nov.
Key findings
- Sporanaerobacter acetigenes is a novel acetogenic, facultatively sulfur-reducing bacterium isolated from an anaerobic wastewater treatment reactor
- The organism is a moderately thermophilic, spore-forming anaerobe with optimal growth at 40°C and pH 7.4
- Phylogenetically, the strain belongs to cluster XII of the Clostridiales and shares 93% 16S rRNA sequence similarity with Clostridium hastiforme
- The bacterium produces acetate as the primary end product from glucose fermentation and can reduce elemental sulfur to sulfide
- The strain's unique combination of sugar fermentation and sulfur reduction distinguishes it from other cluster XII members, warranting a new genus designation
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Abstract
G. Hernandez-Eugenio, M. L. Fardeau, J. L. Cayol, BKC. Patel, P. Thomas, H. Macarie, J. L. Garcia and B. Ollivier
IRD, UR 101 Extremophiles, IFR-BAIM, Universites de Provence et de la Mediterranee, ESIL case 925, 163 avenue de Luminy, 13288 Marseille cedex 09, France
A strictly anaerobic, moderately thermophilic, sporulating rod, designated strain Lup 33(T), was isolated from an upflow anaerobic sludge blanket (UASB) reactor in Mexico. Strain Lup 33(T) possessed a few laterally inserted flagella, had a DNA G+C content of 32.2 mol% and grew optimally at pH 7.4 and 40 degrees C. Growth was observed at temperatures of up to 50 degrees C and was inhibited in the presence of 5% NaCl. Strain Lup 33(T) is heterotrophic and utilized some sugars, peptides and various single amino acids. Gelatin and casein were not used as energy sources. It performed the Stickland reaction and reduced elemental sulfur to sulfide. Acetate was the only fatty acid detected from glucose fermentation, whereas acetate together with isobutyrate and isovalerate were found as end products from peptone fermentation. Phylogenetically, strain Lup 33(T) branched with members of cluster XII of the order Clostridiales, with Clostridium hastiforme as the closest relative (similarity of 93%). On the basis of the phenotypic, genotypic and phylogenetic characteristics of the isolate, it is proposed as a novel species of a new genus, Sporanaerobacter acetigenes gen. nov., sp. nov. The type strain is strain Lup 33(T) (=DSM 13106(T)=CIP 106730(T)).