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Research Article

Aestuariimicrobium kwangyangense gen. nov., sp. nov., an LL-diaminopimelic acid-containing bacterium isolated from tidal flat sediment

Jung, Seo-Youn, Kim, Hee-Sik, Song, Jae Jun, Lee, Seung-Goo, Oh, Tae-Kwang, Yoon, Jung-Hoon

International Journal of Systematic and Evolutionary Microbiology 2007; 57(9):2114 · https://doi.org/10.1099/ijs.0.64917-0

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Summary auto-generated

Researchers isolated four bacterial strains (R27T, R44, R45, and R47) from diesel oil-degraded enrichment cultures derived from oil-contaminated tidal flat sediment in Kwangyang, Korea. Using polyphasic taxonomic analysis combining phenotypic, phylogenetic, and genetic approaches, the strains were characterized as Gram-positive, catalase-positive, non-motile short rods or cocci. The bacteria contained LL-diaminopimelic acid in their cell-wall peptidoglycan, MK-9(H4) as the predominant menaquinone, and anteiso-C15:0 as the major fatty acid. 16S rRNA gene sequence analysis showed the four strains form a distinct evolutionary lineage within the family Propionibacteriaceae with less than 94.5% sequence similarity to related genera. DNA-DNA reassociation values of 91.2-97.3% confirmed the strains represent the same genomic species. The DNA G+C content was 68.8-69.2 mol%. Based on their chemotaxonomic distinctiveness and phylogenetic position, these strains are classified as representatives of a novel genus and species, Aestuariimicrobium kwangyangense gen. nov., sp. nov., with R27T designated as the type strain.

Key findings

  • Four bacterial strains isolated from oil-contaminated tidal flat sediment constitute a new genus and species, Aestuariimicrobium kwangyangense, within family Propionibacteriaceae
  • The strains are Gram-positive, non-motile, contain LL-diaminopimelic acid in peptidoglycan, MK-9(H4) menaquinone, and anteiso-C15:0 as the major fatty acid with DNA G+C content of 68.8-69.2 mol%
  • 16S rRNA gene sequence similarity values less than 94.5% with related genera and DNA-DNA reassociation of 91.2-97.3% confirm the four strains represent a single novel genomic species
  • The bacteria exhibit aerobic metabolism, catalase-positive activity, optimal growth at 35-37°C and pH 7.5-8.5, and can utilize various carbohydrates as carbon sources

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Abstract

Four Gram-positive, catalase-positive, short rod- or coccoid-shaped bacterial strains, R27T, R44, R45 and R47, were isolated from an enrichment culture with diesel oil-degradation activity and their taxonomic positions were investigated using a polyphasic approach. Phenotypic, phylogenetic and genetic similarities indicated that strains R27T, R44, R45 and R47 belong to the same species. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains form a distinct evolutionary lineage within the family Propionibacteriaceae. The novel four strains had cell-wall peptidoglycan based on LL-diaminopimelic acid, MK-9(H4) as the predominant menaquinone and anteiso-C15 : 0 as the major cellular fatty acid. The DNA G+C contents were 68.8–69.2 mol%. These chemotaxonomic properties, together with phylogenetic distinctiveness, distinguish the four novel strains from recognized members of the family Propionibacteriaceae. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strains R27T, R44, R45 and R47 are classified as representatives of a new genus and novel species, Aestuariimicrobium kwangyangense gen. nov., sp. nov., within the family Propionibacteriaceae. The type strain of Aestuariimicrobium kwangyangense sp. nov. is R27T (=KCTC 19182T=JCM 14204T).