Summary auto-generated
Researchers isolated nine bacterial strains from cultured spotted rose snapper (Lutjanus guttatus) in Mexico and characterized them using multiple molecular and phenotypic methods. rep-PCR analysis showed the strains were highly homogeneous (>94% similarity) and recovered from different organs and individuals of the same host species. Sequencing of 16S rRNA genes revealed the strains belonged to the genus Vibrio and were most closely related to Vibrio brasiliensis and Vibrio hepatarius, with sequence similarities of 97.9-98.1% and 97.4-97.8% respectively. DNA-DNA hybridization values with these reference species ranged from 36.8-49.7%, well below the 70% threshold for species delineation. Phenotypic characterization confirmed the strains possessed typical Vibrio traits and displayed characteristics distinguishing them from related species, including positive arginine dihydrolase activity and negative lysine and ornithine decarboxylase activity. Based on combined genotypic and phenotypic evidence, the authors propose a novel species, Vibrio sinaloensis sp. nov., with strain CAIM 797T designated as the type strain.
Key findings
- Nine Vibrio strains isolated from spotted rose snapper in Mexico represent a novel species, Vibrio sinaloensis sp. nov., with type strain CAIM 797T
- 16S rRNA sequence analysis showed 97.9-98.1% similarity to V. brasiliensis and 97.4-97.8% similarity to V. hepatarius, with DNA-DNA hybridization values of 36.8-49.7%, confirming separate species status
- rep-PCR fingerprinting demonstrated high homogeneity among the nine strains (>94% similarity), indicating they form a cohesive genomic group isolated from the same host and geographic location
- Phenotypic characterization identified distinctive traits including positive arginine dihydrolase and tryptophan deaminase activity and negative lysine and ornithine decarboxylase activity that differentiate V. sinaloensis from phylogenetically related Vibrio species
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Abstract
1 CIAD, A.C., Mazatlán Unit for Aquaculture, AP. 711 Mazatlán, Sinaloa 82000, Mexico
2 Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, Spain
3 Departamento de Microbiología y Ecología, Universitat de València, Spain
4 Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain
5 Institut de Reçerca i Tecnologia Agroalimentaries, Centre d'Aquicultura, AP 200 Sant Carles de la Rapita 43540, Spain