Summary auto-generated
This study describes the characterization and formal description of a novel bacterial strain, Gp_4_7.1T, isolated from the cold-water marine sponge Isops phlegraei collected from the Sula Ridge off Norway. The bacterium is a Gram-negative, motile spiral-shaped cell with a single polar flagellum. It is aerobic, oxidase-positive, and catalase-negative, with optimal growth at 20-30°C and pH 7-8 in 2-3% salt concentration. The isolate shows restricted nutritional capacity, utilizing only arabinose among tested substrates, but exhibits lipase activity. Phylogenetic analysis of 16S rRNA gene sequences places the strain within the class Gammaproteobacteria, order Oceanospirillales, with closest relationship to Oleispira antarctica at 93% sequence similarity, but less than 94% similarity to any validly named species. The DNA G+C content is 62.6 mol%, and dominant cellular fatty acids are C16:1ω7 and C16:0. Based on distinct phenotypic, chemotaxonomic, and genetic characteristics, the authors propose this strain as a novel species in a new genus: Spongiispira norvegica gen. nov., sp. nov., with type strain Gp_4_7.1T.
Key findings
- A novel bacterial species, Spongiispira norvegica, was isolated from the cold-water sponge Isops phlegraei off the Norwegian coast and represents both a new genus and species
- The bacterium is a motile Gram-negative spirillum that is aerobic, oxidase-positive, catalase-negative, with restricted nutritional profile utilizing only arabinose among tested substrates
- Phylogenetic analysis based on 16S rRNA gene sequences places the strain in order Oceanospirillales, class Gammaproteobacteria, with <94% sequence similarity to nearest validly named relatives
- The strain has a DNA G+C content of 62.6 mol% and major fatty acids C16:1ω7 (39.4%) and C16:0 (29.7%), differing from the related species Oleispira antarctica
- This represents one of the few Oceanospirillales bacteria associated with sponges and the first isolation from a cold-water boreal sponge species
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Abstract
1 Berlin University of Technology, Environmental Microbiology Group, Franklinstrasse 29, Sekr. FR 1-2, D-10587 Berlin, Germany
2 Hamburg University of Technology, Technical Microbiology, Kasernenstrasse 12, D-21073 Hamburg, Germany
3 University of Hamburg, Institute of Biogeochemistry and Marine Chemistry, Bundesstrasse 55, D-20146 Hamburg, Germany
4 Research Center Ocean Margins, University of Bremen, Klagenfurter Strasse, D-28359 Bremen, Germany
5 Anagnostec GmbH, Am Mühlenberg 11, D-14476 Potsdam-Golm, Germany
6 Berlin University of Technology, Biochemistry and Molecular Biology Group, Franklinstrasse 29, Sekr. OE2, D-10587 Berlin, Germany
7 Novozymes A/S, Bacterial Screening, Krogshøjvej 36, DK-2880 Bagsværd, Denmark
8 Max-Planck Institute for Molecular Genetics, Ihnestrasse 63-73, D-14195 Berlin, Germany