Peptostreptococcus russellii sp. nov., isolated from a swine-manure storage pit

Terence R. Whitehead, Michael A. Cotta, Enevold Falsen, Edward Moore, Paul A. Lawson

¹Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N University Street, Peoria, IL 61604, USA

²Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-41346 Göteborg, Sweden

³Department of Botany and Microbiology, University of Oklahoma, OK 73019, USA

Correspondence
Terence R. Whitehead terry.whitehead{at}ars.usda.gov

International Journal of Systematic and Evolutionary Microbiology 2011; 61(8):1875–1879 · https://doi.org/10.1099/ijs.0.023762-0

Abstract

Using a polyphasic approach, a taxonomic study was performed on seven strains of an unknown Gram-reaction-positive, non-spore-forming, obligately anaerobic coccus-shaped bacterium, isolated from a swine-manure storage pit. Comparative 16S rRNA gene sequencing confirmed that all seven isolates were highly related to each other and formed a hitherto unknown lineage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism was most closely related to Peptostreptococcus anaerobius CCUG 7835^T and Peptostreptococcus stomatis CCUG 51858^T with 16S rRNA gene sequence similarities of 95.5 and 93.0 %, respectively. The peptidoglycan type of the cell wall was determined to be A4α l-Lys–d-Asp and glucose, xylose and traces of mannose were detected as the cell–wall sugars. Based on biochemical, chemotaxonomic and phylogenetic evidence the unknown bacterium represents a new species of the genus Peptostreptococcus, for which the name Peptostreptococcus russellii sp. nov, is proposed. The type strain is RT-10B^T ( = CCUG 58235^T = NRRL B-59380^T = DSM 23041^T).