SGM Journals
Firmicutes And Related Organisms

Lactobacillus faecis sp. nov., isolated from animal faeces

Akihito Endo, Tomohiro Irisawa, Yuka Futagawa-Endo, Seppo Salminen, Moriya Ohkuma, Leon Dicks

  • 1Functional Foods Forum, University of Turku, 20014 Turku, Finland
  • 2Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa
  • 3Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Centre, 305-0074 Ibaraki, Japan
  • Correspondence
    Akihito Endo pegaman{at}hotmail.co.jp

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 12):4502–4507 · https://doi.org/10.1099/ijs.0.052985-0

    View at publisher PubMed

    Abstract

    Three lactic acid bacteria were isolated from faeces of a jackal (Canis mesomelas) and raccoons (Procyron lotor). The isolates formed a subcluster in the Lactobacillus salivarius phylogenetic group, closely related to Lactobacillus animalis, Lactobacillus apodemi and Lactobacillus murinus, by phylogenetic analysis based on 16S rRNA and recA gene sequences. Levels of DNA–DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. The three strains were non-motile, obligately homofermentative and produced l-lactic acid as the main end-product from d-glucose. The strains metabolized raffinose. The major cellular fatty acids in the three strains were C16 : 0, C18 : 1ω9c and C19 : 1 cyclo 9,10. Based on the data provided, it is concluded that the three strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus faecis sp. nov. is proposed. The type strain is AFL13-2T ( = JCM 17300T = DSM 23956T).

    • ↵† These authors contributed equally to this work.

    • The GenBank/EMBL/DDBJ accession numbers for the sequences of 16S rRNA genes and recA genes of Lactobacillus faecis AFL13-2T, AFL18-5 and AFL19-3 are AB812750–AB812752 and AB812753–AB812755, respectively.

    • Two supplementary figures are available with the online version of this paper.