Research Article

Characterisation of a new isolate of Mycobacterium shimoidei from Finland

Journal of Medical Microbiology 2000; 49(10):937

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Summary auto-generated

This research article examines the chemical composition and antimicrobial properties of essential oils from two Salvia species: Salvia officinalis (common sage) and Salvia triloba (Greek sage). The researchers used gas chromatography-mass spectrometry to identify volatile compounds in the essential oils and evaluated their antimicrobial activity against various bacterial and fungal species. The study found that both sage species contain similar major volatile components, including α-thujone, β-thujone, and 1,8-cineole. The essential oils demonstrated varying degrees of antimicrobial activity, with some strains showing greater susceptibility than others. The antimicrobial efficacy appeared related to the concentration and composition of specific volatile compounds. These findings suggest that sage essential oils possess bioactive properties that could have applications in food preservation, herbal medicine, or pharmaceutical development, though the exact mechanisms of action and optimal conditions for use require further investigation.

Key findings

  • Gas chromatography analysis identified α-thujone, β-thujone, and 1,8-cineole as major volatile components in both Salvia officinalis and Salvia triloba essential oils
  • Both sage essential oils demonstrated antimicrobial activity against tested bacterial and fungal species, with varying degrees of effectiveness depending on the microorganism
  • The antimicrobial potency of the essential oils correlated with their chemical composition and concentration of specific volatile compounds
  • Salvia species essential oils show potential for applications in food preservation and herbal medicine based on their antimicrobial properties

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Abstract

This report describes the first isolation of Mycobacterium shimoidei in Finland from a sputum specimen obtained from an elderly female patient. M. shimoidei, a potential lung pathogen, is difficult to identify by routine methods and only a few cases have been reported. The present study demonstrated that M. shimoidei has a characteristic pattern for fatty acids and alcohols in gas liquid chromatography. This chromatogram and the pattern of mycolic acids on thin-layer chromatography allow it to be distinguished routinely. The unique sequence of the 16S rRNA gene and the 16S23S rDNA spacer region allows identification by molecular methods.