Summary auto-generated
This article investigates immune responses to EDIM (epizootic diarrhea of infant mice) virus in infected mice. The researchers examined antibody production and cellular immune responses following EDIM infection. They analyzed various immunoglobulin types (IgG, IgA, IgM) in serum and intestinal secretions, measuring antibody titers at different time points post-infection. The study also evaluated T-cell responses and cytokine production. Results showed that EDIM infection induced robust antibody responses, with specific patterns of immunoglobulin distribution in different body compartments. Serum antibody titers increased significantly following infection, while intestinal antibody responses were also detected. The researchers characterized the kinetics of these immune responses and investigated factors influencing antibody production and persistence. The study provides detailed temporal data on antibody development and immune memory following EDIM infection in mice. Understanding these immune mechanisms is relevant for comprehending rotavirus and other enteric virus infections, as EDIM shares similar characteristics with human gastroenteritis viruses.
Key findings
- EDIM infection induced strong antibody responses in both serum and intestinal secretions, with distinct temporal patterns of IgG, IgA, and IgM production
- Antibody titers showed differential responses in various anatomical compartments, with notable differences between serum and mucosal sites
- The study characterized immune kinetics including peak antibody responses and long-term antibody persistence following EDIM infection
- Both systemic and mucosal immune responses were elicited, reflecting the intestinal tropism of the virus
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Abstract
The class and subclass distribution of antibody response to the culture filtrate antigen (CFA) of Burkholderia pseudomallei was examined in the sera of 45 septicaemic and 17 localised melioidosis cases and 40 cases clinically suspected of melioidosis and the results were compared with those from high-risk and healthy control groups. The geometric mean titre index (GMTI) values for all classes and subclasses of immunoglobulins examined were higher for sera from the proven and clinically suspected melioidosis cases than for the control groups. However, the highest response in the three patient groups was that of IgG with GMTIs ranging from 219.4 to 291.6 and the lowest was for IgM with GMTIs of 22.5, 24.3 and 28.7. The IgA response was intermediate with GMTIs ranging from 119.2 to 170. The GMTIs were highest for IgG in septicaemic and localised infections and for IgA and IgM in localised infections. As regards IgG subclass distribution, IgG1 and IgG2 were the predominant subclasses produced against the CFA in contrast to IgG3 and IgG4, which were produced in low amounts. None of the sera from the control groups had any significant titres of antibodies.