Abstract
Infectious pancreatic necrosis (IPN) virus is the causal agent of a highly contagious and destructive disease of young hatchery-reared trout (Wolf et al. 1960). Studies with metabolic inhibitors indicated that the virus nucleic acid is RNA (Malsberger & Cerini, 1965) and electron microscopy showed that the virus particles had a diameter of approximately 65 nm. and resembled those of reovirus (Moss & Gravell, 1969; Lightner & Post, 1969). This paper presents the results of a study of macromolecular synthesis in rainbow trout gonad (RTG) cells infected with IPN virus.
The continuous rainbow trout gonad cell line (RTG-2) (Wolf & Quimby, 1962) was propagated at 22° in growth medium (GM) consisting of Eagle's minimum essential medium with Earle's balanced salt solution (EBSS) and supplemented with 10% foetal bovine serum. Maintenance medium (MM) was the same except for the serum concentration which was reduced to 2%. Both GM and MM contained 100 units of penicillin, 100 µg. of streptomycin and 25 units of mycostatin per ml.