Research Article

The Relation Between Radiation Stability and DNA Replication of Phage T4

Journal of General Virology 1970; 8(2):121 · https://doi.org/10.1099/0022-1317-8-2-121

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Abstract

First, samples taken from the infected cells at different times were irradiated with ultraviolet light in order to obtain a set of Luria-Latarjet curves. Secondly, and with the same culture of infected cells, the kinetics of phage DNA synthesis was followed using a density gradient technique.

A comparison of these results suggests that the radiation stability shown by the Luria-Latarjet curves is first detectable at about the time that phage DNA synthesis commences. The radiation stability, however, increases at a rate which is much faster than the rate of replication of the parental phage DNA.

This work was carried out when both authors were members of the Medical Research Council Microbial Genetics Research Unit, then at Hammersmith Hospital, London, W.12.