Summary auto-generated
This study used 16S rRNA gene sequencing and phylogenetic analysis to resolve the taxonomic classification of the heterogeneous genus Thermomonospora and related actinomycetes. Seven Thermomonospora species were sequenced and compared with members of the suborder Streptosporangineae. The analysis revealed that Thermomonospora species were phylogenetically associated with distinct groups of actinomycetes. Thermomonospora formosensis grouped with Actinomadura species, Thermomonospora mesophila clustered with Microbispora species, and Thermomonospora fusca, alba, and mesouviformis formed a coherent clade more closely related to Nocardiopsis. Additionally, the genus Microtetraspora was found to be heterogeneous, with the original species (glauca, fusca, niveoalba) and the Microtetraspora pusilla group forming two distinct phylogenetic clades. Based on these molecular, chemotaxonomic, and phenotypic findings, the authors propose significant reclassifications: transferring Thermomonospora formosensis to Actinomadura, Thermomonospora mesophila to Microbispora, and creating the new genus Thermobifida for Thermomonospora fusca and alba. The Microtetraspora pusilla group is transferred to the new genus Nonomuria.
Key findings
- Thermomonospora species belong to phylogenetically distinct groups requiring reclassification into separate genera based on 16S rRNA sequence analysis
- The new genus Thermobifida gen. nov. is established for Thermomonospora fusca and T. alba, placed in family Nocardiopsaceae rather than Thermomonosporaceae
- Thermomonospora mesophila is reclassified to Microbispora based on identical chemotaxonomic properties and high 16S rRNA sequence similarity
- The genus Microtetraspora is heterogeneous, with 15 species of the Microtetraspora pusilla group transferred to the new genus Nonomuria gen. nov.
- Chemotaxonomic characteristics including cell wall type, menaquinone profiles, phospholipid patterns, and fatty acid types support the proposed reclassifications
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Abstract
Almost complete 16S rRNA sequences from seven Thermomonospora strains, Thermomonospora curvata, Thermomonospora formosensis, Thermomonospora fusca, Thermomonospora mesophila, Thermomonospora chromogena, Thermomonospora alba and Thermomonospora mesouviformis (a synonym of Thermomonospora alba) were determined and subjected to phylogenetic analysis together with the sequences from all the representative members of the suborder Streptosporangineae. On the basis of phylogenetic, chemotaxonomic and phenotypic evidence, the transfer is proposed of Thermomonospora formosensis to the genus Actinomadura as Actinomadura formosensis comb. nov., Thermomonospora mesophila to the genus Microbispora as Microbispora mesophila comb. nov., and Thermomonospora fusca and Thermomonospora alba to a new genus, Thermobifida gen. nov., which belongs to the family Nocardiopsaceae, as Thermobifida fusca comb. nov. and Thermobifida alba comb. nov. Thermobifida alba is designated the type species of the genus. The transfer is also proposed of all species of the Microtetraspora pusilla group, which were transferred from Actinomadura, to a new genus, Nonomuria gen. nov., as Nonomuria africana comb, nov., Nonomuria angiospora comb. nov., Nonomuria fastidiosa comb. nov., Nonomuria ferruginea comb. nov., Nonomuria flexuosa comb. nov., Nonomuria helvata comb. nov., Nonomuria polychroma comb. nov., Nonomuria pusilla comb. nov., Nonomuria recticatena comb. nov., Nonomuria roseola comb. nov., Nonomuria roseoviolacea comb. nov., Nonomuria rubra comb. nov., Nonomuria salmonea comb. nov., Nonomuria spiralis comb. nov. and Nonomuria turkmeniaca comb. nov. Nonomuria pusilla is designated the type species of the genus.