Summary auto-generated
Researchers isolated an alkaliphilic, endospore-forming bacterium from Brazilian soil and characterized it as a new species within the genus Paenibacillus. Strain 324T was distinguished by its ability to grow optimally at pH 10 and 40°C, unlike other Paenibacillus species that prefer neutral pH and lower temperatures. The organism produces cyclodextrin from starch via cyclodextrin glycosyltransferase. Chemotaxonomic analysis revealed a DNA G+C content of 50.9 mol%, meso-diaminopimelic acid in the cell wall peptidoglycan, MK-7 as the predominant menaquinone, and anteiso-C15:0 as the major fatty acid. Phylogenetic analysis using 16S rDNA sequencing showed 90.6-95.9% similarity to other Paenibacillus species, with the strain occupying a distinct evolutionary position within the genus. Based on combined phenotypic, chemotaxonomic, and phylogenetic data, the authors propose strain 324T as a new species, Paenibacillus campinasensis, named after Campinas, Brazil.
Key findings
- Paenibacillus campinasensis is an alkaliphilic bacterium with optimum growth at pH 10, distinctly different from other Paenibacillus species that grow optimally at neutral pH
- The organism grows optimally at 40°C and produces cyclodextrin from starch using cyclodextrin glycosyltransferase
- 16S rDNA phylogenetic analysis confirms strain 324T as a distinct Paenibacillus species with 90.6-95.9% similarity to known species
- Chemotaxonomic properties including 50.9 mol% G+C content, meso-diaminopimelic acid cell wall, and anteiso-C15:0 fatty acids support classification within Paenibacillus genus
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Abstract
An alkaliphilic, endospore-forming bacterium isolated from Brazilian soil was taxonomically studied and is proposed as a new Paenibacillus species. This organism (strain 324T) was particularly distinguishable from other Paenibacillus species by its ability to grow optimally at pH 10 and 40 °C. The DNA G+C content was 50-9 mol%. The diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. MK-7 was the predominant menaquinone and anteiso-C15:0 was the major fatty acid. Levels of 16S rDNA similarity between strain 324Tand other Paenibacillus species were 90-6-95-9%. Phylogenetically, strain 324Tformed an evolutionary lineage distinct from other species within the evolutionary radiation encompassing the genus Paenibacillus. Based on phenotypic and chemotaxonomic properties, and phylogenetic inference, it is proposed that strain 324Tshould be placed in the genus Paenibacillus as a new species, Paenibacillus campinasensis. The type strain of the new species is strain 324T(= KCTC 0364BPT).