Summary auto-generated
This study describes the discovery and characterization of a new bacterial species, Ignavigranum ruoffiae, isolated from two human clinical specimens—a wound infection and an ear abscess. Using a combination of phenotypic and molecular methods, researchers demonstrated that the two strains were genealogically identical. Comparative 16S rRNA gene sequencing revealed that this organism is phylogenetically most closely related to Facklamia hominis, though distinct enough to warrant classification as a new genus. The bacterium is a Gram-positive, catalase-negative, facultatively anaerobic coccus with distinctive characteristics including weak acid production from glucose but minimal fermentation of other carbohydrates. Cell wall analysis showed an L-lysine-directly cross-linked murein structure. Biochemical testing and whole-cell protein electrophoresis further distinguished it from related genera Facklamia and Globicatella. Based on phylogenetic evidence and phenotypic traits, the authors propose the new genus and species Ignavigranum ruoffiae, with the type strain CCUG 37658T.
Key findings
- Two human clinical isolates constitute a new bacterial genus and species, Ignavigranum ruoffiae, based on 16S rRNA gene sequencing and polyphasic taxonomic analysis
- The organism is phylogenetically most closely related to Facklamia hominis (84% bootstrap support, 8.2% sequence divergence) but distinct enough to merit separate generic status
- Ignavigranum ruoffiae is a Gram-positive, catalase-negative, facultatively anaerobic coccus with characteristic asaccharolytic phenotype and L-lysine-direct cross-linked murein (type A1a)
- Whole-cell protein electrophoresis confirmed the two isolates form a tight cluster distinctly separate from related organisms including Facklamia and Globicatella species
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Abstract
Two strains of a hitherto undescribed Gram-positive catalase-negative, facultatively anaerobic coccus isolated from human sources were characterizec by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated the unknown strains were genealogically identical, and constitute a new line close to, but distinct from, the genera Facklamia and Globicatella. The unknown bacterium was readily distinguished from Facklamia species and Globicatella sanguinus by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium be classifiec as lgnavigranum ruoffiae gen. nov., sp. nov. The type strain of lgnavigranum ruoffiae is CCUG 37658T.