Abstract
Four new yeasts are described that were recognized as novel from nucleotide substitutions in domain D1/D2 of 26S rDNA, a region that is sufficiently divergent to allow resolution of most ascomycetous yeast species. The new species and their type strains are as follows: Pichia maclurae NRRL Y-5377(T) (=CBS 8671(T)); Pichia misumaiensis NRRL Y-17389(T) (=CBS 8062(T)); Candida mycetangii NRRL Y-6843(T) (=CBS 8675(T)); and Candida ulmi NRRL YB-2694(T) (=CBS 8670(T)). The two Pichia species form spherical ascospores and are heterothallic. Phylogenetic analysis of domain D1/D2 sequences placed the four new species in the Pichia anomala clade.