Abstract
MC Macian, W Ludwig, R Aznar, PAD Grimont, KH Schleifer, E Garay and MJ Pujalte
Departamento de Microbiologia y Ecologia, Universitat de Valencia, Campus de Burjassot, E-46100 Valencia, Spain
Twelve phenotypically similar marine bacteria have been studied by means of ribotyping, DNA--DNA hybridization and cultural and physiological characterization. Phylogenetic analysis has been performed of the 16S and 23S rRNA genes of two representative strains. Phylogenetically, they belong to the Vibrio/Photobacterium branch of the gamma-Proteobacteria and they share all of the properties that define the genus Vibrio. The strains represent a new Vibrio species that is phenotypically similar to Vibrio splendidus. However, resistance to the vibriostatic agent O129 and production of acid from several carbohydrates allow differentiation between V. splendidus and the proposed new species. The DNA G+C content of the proposed type strain is 44.0 mol%. The name Vibrio lentus sp. nov. is proposed for the new species and strain 4OM4(T) (=CECT 5110(T)=DSM 13757(T)) is the type strain.