Summary auto-generated
Researchers isolated three yeast strains from plant leaves collected in a tropical rain forest in Thailand. Two strains were identified as known Kockovaella species: K. sacchari and K. thailandica. The third strain, designated TY-278T, was found to represent a previously undescribed species. This new isolate was characterized by the production of stalked conidia and ballistoconidia (spore-like structures), inability to ferment sugars, positive urease and diazonium blue B reactions, the presence of xylose in cell wall components, and Q-10 as its major ubiquinone. Phylogenetic analysis of 18S rRNA and internal transcribed spacer (ITS) sequences grouped the isolate with other Kockovaella species. DNA complementarity experiments demonstrated genetic distinctness from all known Kockovaella species. Based on morphological, biochemical, and molecular phylogenetic characteristics, the authors formally described this isolate as Kockovaella barringtoniae sp. nov., named after Barringtonia sp., the plant from which it was isolated. The type strain TY-278T has been deposited in microbial culture collections as JCM 10998T and TISTR 5770T.
Key findings
- A new basidiomycetous yeast species, Kockovaella barringtoniae, was isolated from a Barringtonia plant leaf in a Thai tropical rain forest
- The new species produces distinctive stalked conidia and ballistoconidia and is unable to ferment sugars, distinguishing it from related Kockovaella species
- DNA complementarity assays showed the isolate shares only 1-10% homology with known Kockovaella species, confirming its status as a distinct species
- Phylogenetic analysis of 18S rRNA and ITS sequences placed K. barringtoniae within the Kockovaella genus cluster with strong statistical support
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Abstract
One yeast strain characterized by the proliferation of non-ballistosporous stalked conidia, budding cells and ballistoconidia, the presence of xylose in whole-cell hydrolysates, the presence of Q-10 as the major ubiquinone isoprenologue, the inability to ferment sugars and positive diazonium blue B (DBB) and urease reactions was isolated from a plant sample collected in a tropical rain forest in Thailand. The isolate clustered with Kockovaella species in the 18S rDNA-based tree. On the basis of the morphological, biochemical and molecular phylogenetic characteristics, the isolate was assigned to the genus Kockovaella. DNA complementarity experiments showed that the isolate was genetically distinct from known species of the genus Kockovaella. The isolate is described as Kockovaella barringtoniae sp. nov. The type strain is strain TY-278(T) (= JCM 10998(T)=TISTR 5770(T)).