Summary auto-generated
This study reclassifies the bacterium Desulfotomaculum auripigmentum as Desulfosporosinus auripigmenti based on phylogenetic, morphological, and chemotaxonomic evidence. The type strain DSM 13351T was originally affiliated with Desulfotomaculum based on 16S rRNA analysis, but subsequent longer sequence analysis (1532 bases versus the original 1263 bases) revealed 97.4-97.9% similarity to Desulfosporosinus species rather than Desulfotomaculum. DNA-DNA hybridization values below 30% confirmed genomic distinctness from other Desulfosporosinus species. Morphological examination revealed that the strain does form oval, subterminal to terminal spores—contrary to the original description. The organism possesses LL-diaminopimelic acid and MK-7 as the predominant menaquinone, with characteristic even-carbon fatty acids and dimethylacetals. The reclassified species differs from other Desulfosporosinus members by its non-motility, smaller cell diameter, and ability to utilize malate and glycerol as electron donors and fumarate and arsenate as electron acceptors. This reclassification necessitated an emended genus description of Desulfosporosinus.
Key findings
- Desulfotomaculum auripigmentum is reclassified as Desulfosporosinus auripigmenti based on phylogenetic analysis showing 97.4-97.9% 16S rRNA gene similarity to Desulfosporosinus species
- The strain forms oval, subterminal to terminal spores, contrary to the original description, and possesses LL-diaminopimelic acid and MK-7 menaquinone
- DNA-DNA hybridization values below 30% with other Desulfosporosinus species confirm it maintains distinct species status
- D. auripigmenti is differentiated from other Desulfosporosinus species by non-motility, smaller cell diameter (0.4 μm), and unique metabolic capabilities with malate, glycerol, fumarate, and arsenate
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Abstract
The species Desulfotomaculum auripigmentum is reclassified as Desulfosporosinus auripigmenti corrig., comb. nov. on the basis of morphological and physiological traits, phylogenetic position and chemotaxonomic properties. Characteristics supplementary to those provided in the original description reveal that the type strain, DSM 13351T (=ATCC 700205T), forms oval, subterminal to terminal spores, possesses LL-diaminopimelic acid and contains MK-7 as the predominant menaquinone, while the whole-cell methanolysate contains even-carbon, straight-chain saturated and mono-unsaturated fatty acids and 1,1-dimethylacetals as major components. DNADNA reassociation values below 30 % for Desulfosporosinus orientis DSM 765T and Desulfosporosinus meridiei DSM 13257T demonstrate that strain DSM 13351T shows sufficient genomic differences to maintain its species status. Lack of motility, a smaller cell diameter and the ability to use malate and glycerol as electron donors and fumarate and arsenate as electron acceptors are the main properties that differentiate Desulfosporosinus auripigmenti from the other two species of the genus.