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Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant

Aslam, Zubair, Im, Wan-Taek, Kim, Myung Kyum, Lee, Sung-Taik

International Journal of Systematic and Evolutionary Microbiology 2005; 55(2):747 · https://doi.org/10.1099/ijs.0.63459-0

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Summary auto-generated

Researchers isolated a novel bacterial strain designated Kw05T from anaerobic granules in a beer-brewing wastewater treatment plant in Korea. Using polyphasic taxonomy combining 16S rRNA gene sequencing, chemotaxonomic analysis, and phenotypic characterization, they determined this Gram-negative, rod-shaped bacterium belongs to the genus Flavobacterium but represents a previously undescribed species. The strain showed less than 97% 16S rRNA gene sequence similarity to known Flavobacterium species, with closest relatives being F. limicola (96.6%), F. hibernum (96.3%), F. hydatis (96.1%), and F. xinjiangense (96.1%). Genomic DNA G+C content was 36.2 mol%, consistent with the genus. Major cellular fatty acids included iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH, and iso-C17:1ω9c, with MK-6 as the primary quinone. The aerobic, heterotrophic strain grew at 15-37°C and could utilize glucose, mannose, N-acetylglucosamine, maltose, propionate, and L-proline as sole carbon sources. Based on these findings, the authors propose the name Flavobacterium granuli sp. nov., with strain Kw05T as the type strain.

Key findings

  • Strain Kw05T represents a novel Flavobacterium species isolated from anaerobic treatment granules, distinguishable by <97% 16S rRNA gene sequence similarity to all recognized Flavobacterium species
  • The bacterium is Gram-negative, aerobic, non-motile, and yellow-pigmented, growing optimally at 25-30°C with a genomic DNA G+C content of 36.2 mol%
  • Chemotaxonomic profile includes MK-6 as the major quinone and distinctive fatty acid composition dominated by iso-C15:0 (28.2%)
  • The strain exhibits limited substrate utilization, utilizing only glucose, mannose, N-acetylglucosamine, maltose, propionate, and L-proline as sole carbon sources
  • Proposed species name is Flavobacterium granuli sp. nov., with type strain Kw05T (=KCTC 12201T=IAM 15099T)

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Abstract

A Gram-negative, rod-shaped, non-spore-forming bacterium (designated strain Kw05T) was isolated from granules used in the wastewater treatment plant of a beer-brewing factory in Kwang-Ju, Republic of Korea. On the basis of 16S rRNA gene sequence similarity, strain Kw05T was shown to belong to the family Flavobacteriaceae, and was most closely related to Flavobacterium limicola (96·6 %), Flavobacterium hibernum (96·3 %), Flavobacterium hydatis (96·1 %) and Flavobacterium xinjiangense (96·1 %). The G+C content of the genomic DNA of strain Kw05T was 36·2 mol%, within the range of 3237 mol% for the genus Flavobacterium. Chemotaxonomic data (major menaquinone MK-6; major fatty acids iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and iso-C17 : 1ω9c) supported the classification of strain Kw05T within the genus Flavobacterium. Kw05T therefore represents a novel species, for which the name Flavobacterium granuli sp. nov. is proposed. The type strain is Kw05T (=KCTC 12201T=IAM 15099T).