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Shinella granuli gen. nov., sp. nov., and proposal of the reclassification of Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp. nov.

An, Dong-Shan, Im, Wan-Taek, Yang, Hee-Chan, Lee, Sung-Taik

International Journal of Systematic and Evolutionary Microbiology 2006; 56(2):443 · https://doi.org/10.1099/ijs.0.63942-0

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Summary auto-generated

Researchers isolated a novel bacterial strain, Ch06T, from an upflow anaerobic sludge blanket reactor and determined its taxonomic position through 16S rRNA gene sequence analysis, physiological testing, and chemotaxonomic characterization. The strain was Gram-negative, aerobic, motile, oxidase- and catalase-positive, with a DNA G+C content of 66 mol%. Phylogenetic analysis showed it formed a distinct cluster with Zoogloea ramigera ATCC 19623, sharing 97.9% 16S rRNA sequence similarity. However, DNA-DNA hybridization revealed only 33% relatedness between the strains, indicating they represent separate species. Both strains possessed ubiquinone-10 as the major respiratory quinone and similar cellular fatty acid profiles dominated by summed feature 7. Based on morphological, phylogenetic, and chemotaxonomic data, the authors established a new genus Shinella and described two species: Shinella granuli gen. nov., sp. nov. (type strain Ch06T) and reclassified Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp. nov. The genus Shinella is characterized by Gram-negative motile rods with multiple polar flagella, catalase and oxidase positivity, predominant fatty acids C16:0 and summed feature 7, and 3-OH C16:0 as the unique hydroxy fatty acid profile. Both species belong to the family Rhizobiaceae within Alphaproteobacteria.

Key findings

  • A novel bacterial strain Ch06T isolated from UASB reactor granules represents a new genus and species, Shinella granuli, established based on phylogenetic, phenotypic, and chemotaxonomic characteristics.
  • Zoogloea ramigera ATCC 19623 was reclassified into the new genus Shinella as Shinella zoogloeoides sp. nov., despite sharing 97.9% 16S rRNA similarity with S. granuli, because DNA-DNA hybridization showed only 33% relatedness.
  • The genus Shinella is characterized by the unique hydroxy fatty acid profile of 3-OH C16:0 and predominant cellular fatty acids including summed feature 7 (C18:1ω7c/ω9t/ω12t), distinguishing it from related genera.
  • Both Shinella species belong to the family Rhizobiaceae within Alphaproteobacteria and contain ubiquinone-10 as their major respiratory quinone, with DNA G+C content of 64-66 mol%.

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Abstract

The taxonomic position of a novel bacterial strain, Ch06T, isolated from an upflow anaerobic sludge blanket reactor was determined. Strain Ch06T was Gram-negative, aerobic, motile and oxidase- and catalase-positive. A comparative 16S rRNA gene sequence analysis showed a clear affiliation of strain Ch06T to the Alphaproteobacteria and it was most closely related to Zoogloea ramigera ATCC 19623 and Mycoplana dimorpha IAM 13154T (97·9 and 96·3 % sequence similarity, respectively). The major respiratory quinone was Q-10 and the predominant fatty acids were C16 : 0, 3-OH C16 : 0, C18 : 0, C19 : 0 cyclo ω8c and summed feature 7 (C18 : 1ω7c/ω9t/ω12t, C18 : 1ω7c/ω9c/ω12t). On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the novel isolate was assigned to a new genus, Shinella gen. nov., as Shinella granuli gen. nov., sp. nov. (type strain Ch06T=KCTC 12237T=JCM 13254T). It is proposed that Zoogloea ramigera ATCC 19623 is reclassified into the novel genus Shinella as Shinella zoogloeoides sp. nov. (type strain ATCC 19623T=IAM 12669T=I-16-MT).