Summary auto-generated
Researchers isolated a novel bacterial strain, CL-ES2T, from coastal water off South Korea's east coast. Phylogenetic analysis of 16S rRNA gene sequences revealed that this Gram-negative, non-motile, strictly aerobic bacterium was related to genera Phaeobacter, Leisingera, and Marinovum within the family Rhodobacteraceae, but formed a distinct evolutionary lineage. The strain grew optimally at 25°C and pH 7, with preference for 3-4% sea salt concentration. Biochemical characterization showed a DNA G+C content of 47.0 mol%, major fatty acid C18:1ω7c, and isoprenoid quinone Q-10. Polar lipids included phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, and several unidentified lipids. Phenotypic and chemotaxonomic differences—including lower G+C content compared to related genera, absence of motility, and positive Tween 80 hydrolysis—distinguished this strain from known genera. Based on polyphasic analysis combining phylogenetic, morphological, and chemical data, the researchers established a novel genus and species, naming it Pelagicola litoralis with type strain CL-ES2T.
Key findings
- Pelagicola litoralis represents a novel genus and species isolated from Korean coastal water, forming a distinct lineage within the Roseobacter clade family Rhodobacteraceae
- The strain is Gram-negative, non-motile, strictly aerobic, club-shaped, with optimal growth at 25°C, pH 7, and 3-4% sea salt concentration
- Chemotaxonomic analysis showed DNA G+C content of 47.0 mol%, major fatty acid C18:1ω7c, isoprenoid quinone Q-10, and distinctive polar lipid composition
- Phenotypic differentiation from related genera Phaeobacter, Leisingera, and Marinovum includes lower G+C content, absence of motility, and positive Tween 80 hydrolysis
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Abstract
A Gram-negative, strictly aerobic, non-motile, club-shaped bacterial strain, designated CL-ES2T, was isolated from coastal water from the east coast of Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain CL-ES2T was related to the genera Phaeobacter (95.0–96.6 % similarity to the type strains), Leisingera (96.1 %) and Marinovum (95.6 %) in the family Rhodobacteraceae. However, strain CL-ES2T did not form a robust clade with any species of the Roseobacter clade, instead forming a distinct subline. The optimum temperature and pH for growth were 25 °C and pH 7. Strain CL-ES2T was able to grow with sea salts at concentrations in the range 2–6 %, with optimum growth occurring at 3–4 %. The major fatty acid was C18 : 1ω7c (75.2 %). The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and three unidentified lipids. The isoprenoid quinone was Q-10. The G+C content of the DNA was 47.0 mol%. On the basis of the data from the polyphasic analysis, strain CL-ES2T represents a novel genus and species, for which the name Pelagicola litoralis gen. nov., sp. nov. is proposed. The type strain of Pelagicola litoralis is CL-ES2T (=KCCM 42274T =DSM 18290T).