SGM Journals
Bacteroidetes

Chryseobacterium viscerum sp. nov., isolated from diseased fish

L. Zamora, A. I. Vela, M. A. Palacios, C. Sánchez-Porro, L. A. Svensson-Stadler, L. Domínguez, E. R. B. Moore, A. Ventosa, J. F. Fernández-Garayzábal

  • 1Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain
  • 2Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
  • 3Piszolla, S. L. 37800 Alba de Tormes, Salamanca, Spain
  • 4Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
  • 5Culture Collection University of Gothenburg (CCUG), Department of Infectious Disease, Sahlgrenska Academy of the University of Gothenburg, 41346 Göteborg, Sweden
  • Correspondence
    A. I. Vela avela{at}vet.ucm.es

    • International Journal of Systematic and Evolutionary Microbiology 2012; 62(Pt 12):2934–2940 · https://doi.org/10.1099/ijs.0.036699-0

    View at publisher PubMed

    Abstract

    A taxonomic study was carried out on five Gram-staining-negative, catalase- and oxidase-positive, rod-shaped bacteria isolated from the gills and livers of five diseased rainbow trout. The five novel isolates were designated strains 687B-08T, 445-08, 452-08, 453B-08 and 967B-08. In phylogenetic analyses based on 16S rRNA gene sequences, the five novel strains appeared almost identical (99.0–100 % sequence similarity) and to belong to the genus Chryseobacterium. Strain 687B-08T (the strain selected to represent the five novel isolates) was found to be most closely related to Chryseobacterium oncorhynchi 701B-08T (98.9 % sequence similarity), Chryseobacterium ureilyticum F-Fue-04IIIaaaaT (98.6 %), Chryseobacterium indologenes ATCC 29897T (98.3 %), Chryseobacterium jejuense JS17-8T (98.1 %) and Chryseobacterium gleum ATCC 35910T (98.1 %). In DNA–DNA hybridizations, DNA–DNA relatedness values of 99–100 % were recorded between the five novel strains. Lower DNA–DNA relatedness values (21–57 %) were recorded between strain 687B-08T and C. oncorhynchi 701B-08T, C. ureilyticum F-Fue-04IIIaaaaT and the type strains of other closely related, established species of the genus Chryseobacterium. The predominant respiratory quinone of strain 687B-08T was MK-6 and the major cellular fatty acids were iso-C15 : 0, iso-C17 : 1ω9c, iso-C17 : 0 3-OH and C16 : 1ω6c. The G+C content of the genomic DNA of strain 687B-08T was 38.6 mol%. Based on the phenotypic and genotypic evidence, the five novel strains isolated from rainbow trout represent a single, novel species of the genus Chryseobacterium, for which the name Chryseobacterium viscerum sp. nov. is proposed. The type strain is 687B-08T ( = CECT 7793T  = CCUG 60103T).

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 687B-08T, 445-08, 452-08, 453B-08 and 967B-08 are FR871426, FR871423, FR871424, FR871425 and FR871427, respectively.