SGM Journals
Bacteroidetes

Flavobacterium xueshanense sp. nov. and Flavobacterium urumqiense sp. nov., two psychrophilic bacteria isolated from glacier ice

Kun Dong, Hongcan Liu, Jianli Zhang, Yuguang Zhou, Yuhua Xin

  • 1CGMCC–China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
  • 2China Agricultural University, Beijing 100193, PR China
  • 3School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China
  • Correspondence
    Yuhua Xin xinyh{at}im.ac.cn

    • International Journal of Systematic and Evolutionary Microbiology 2012; 62(Pt 5):1151–1157 · https://doi.org/10.1099/ijs.0.030049-0

    View at publisher PubMed

    Abstract

    Two Gram-stain-negative, rod-shaped bacteria, designated strains Sr22T and Sr25T, were isolated from water of melted ice from the China No.1 glacier, Xinjiang Uygur Autonomous Region, China. Cells formed yellow, circular, convex colonies. 16S rRNA gene sequence analysis indicated that strains Sr22T and Sr25T belong to the genus Flavobacterium, sharing ≤99.1  and ≤99.6 % similarity, respectively, with the type strains of recognized species of the genus. Strain Sr22T shared highest 16S rRNA gene sequence similarity with Flavobacterium tiangeerense CGMCC 1.6847T (98.6 %), Flavobacterium fryxellicola LMG 22022T (98.1 %) and Flavobacterium omnivorum CGMCC 1.2747T (99.1 %). Strain Sr25T shared highest similarity with Flavobacterium sinopsychrotolerans CGMCC 1.8704T (98.5 %), Flavobacterium degerlachei NBRC 102677T (98.4 %) and Flavobacterium xinjiangense CGMCC 1.2749T (99.5 %). The predominant fatty acids of strain Sr22T were iso-C15 : 1 G (6.01 %), iso-C15 : 0 (8.93 %), iso-C16 : 1 H (12.68 %), iso-C16 : 0 (10.4 %), C15 : 1ω6c (8.97 %), C17 : 1ω6c (5.96 %), iso-C16 : 0 3-OH (11.14 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 12.33 %). The major fatty acids of strain Sr25T were iso-C15 : 0 (10.8 %), anteiso-C15 : 0 (5.23 %), C15 : 1ω6c (11.79 %), C17 : 1ω6c (5.43 %), iso-C16 : 0 3-OH (7.04 %) and summed feature 3 (20.42 %). The genomic DNA G+C contents of strains Sr22T and Sr25T were 37.2 and 35.1 mol%. On the basis of differential phenotypic and phylogenetic characteristics, these strains are considered to represent two novel species of the genus Flavobacterium, for which the names Flavobacterium xueshanense sp. nov. (type strain Sr22T  = CGMCC 1.9227T  = NBRC 106479T) and Flavobacterium urumqiense sp. nov. (type strain Sr25T  = CGMCC 1.9230T  = NBRC 106480T) are proposed.

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains Sr22T and Sr25T are HQ436466 and HQ436467, respectively.

    • A supplementary figure is available with the online version of this paper.