Abstract
A pink-pigmented, facultatively methylotrophic bacterium, strain 35aT, was isolated from the leaves of Oxalis corniculata. Cells of strain 35aT were Gram-reaction-negative, motile, non-spore-forming rods. The highest 16S rRNA gene pairwise sequence similarities for strain 35aT were found with the strains of Methylobacterium iners 5317S-33T (96.7 %), ‘Methylobacterium soli’ YIM 48816 (96.6 %) and Methylobacterium jeotgali S2R03-9T (96.3 %). 16S rRNA gene sequence similarities with the type strains of all other recognized species of the genus Methylobacterium were below 96 %. Major cellular fatty acids were C18 : 1ω7c, C18 : 0 and C16 : 0. The results of DNA–DNA hybridization experiments, analysis of cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS spectral pattern analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 35aT from its nearest phylogenetic neighbours. Strain 35aT is therefore considered to represent a novel species within the genus Methylobacterium, for which the name Methylobacterium oxalidis sp. nov. is proposed. The type strain is 35aT ( = DSM 24028T = NBRC 107715T).
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The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are AB607860 (strain 35aT 16S rRNA gene), AB607859 (strain 35aT partial cpn60 gene), AB640729 (‘Methylobacterium soli’ KCTC 22810 partial cpn60 gene), AB607858 (Methylobacterium iners DSM 19015T partial cpn60 gene) and AB608730 (Methylobacterium jeotgali LMG 23639T partial cpn60 gene).
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Three supplementary figures are available with the online version of this paper.
- Abbreviations:
- MALDI-TOF/MS
- matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- MDH
- methanol dehydrogenase
- PQQ
- pyrroloquinoline quinone