SGM Journals
Firmicutes And Related Organisms

Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov.

Sylvie Cousin, Laurence Motreff, Marie-Laure Gulat-Okalla, Catherine Gouyette, Cathrin Spröer, Peter Schumann, Evelyne Begaud, Christiane Bouchier, Dominique Clermont, Chantal Bizet

  • 1Institut Pasteur, Plate-forme Génomique, Dept Génomes et Génétique, F-75015 Paris, France
  • 2Institut Pasteur, CRBIP (Centre de Ressources Biologiques de l’Institut Pasteur), Dept Microbiologie, F-75015 Paris, France
  • 3DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures), D-38124 Braunschweig, Germany
  • Correspondence
    Sylvie Cousin sylvie.cousin{at}pasteur.fr

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 1):53–59 · https://doi.org/10.1099/ijs.0.036665-0

    View at publisher PubMed

    Abstract

    Strains 1517T and 61DT were characterized by phenotypic and molecular taxonomic methods. These Gram-positive lactic acid bacteria were homo-fermentative, facultatively anaerobic short rods. They were phylogenetically related to the genus Lactobacillus according to 16S rRNA gene sequence analysis, with 99 % similarity between strain 1517T and the type strain of Lactobacillus gigeriorum, and 98.6, 98.5 and 98.4 % between strain 61DT and Lactobacillus gasseri, Lactobacillus taiwanensis and Lactobacillus johnsonii, respectively. Multilocus sequence analysis and metabolic analysis of both strains showed variation between the two strains and their close relatives, with variation in the position of the pheS and rpoA genes. The DNA–DNA relatedness of 43.5 % between strain 1517T and L. gigeriorum, and 38.6, 29.9 and 39.7 % between strain 61DT and L. johnsonii, L. taiwanensis and L. gasseri, respectively, confirmed their status as novel species. Based on phenotypic and genotypic characteristics, two novel species of Lactobacillus are proposed: Lactobacillus pasteurii sp. nov., with 1517T ( = CRBIP 24.76T = DSM 23907T) as the type strain, and Lactobacillus hominis sp. nov., with 61DT ( = CRBIP 24.179T = DSM 23910T) as the type strain.

    • The GenBank/EMBL/DDBJ accession numbers for the hsp60, pheS, rpoA and 16S rRNA gene sequences of strains 1517T, 61DT, CRBIP 24.76T and CRBIP 24.179T are HE573889–HE573890, HE573897–HE573898, HE573905–HE573906 and FR681901–FR681902, respectively. The hsp60, pheS, rpoA and 16S rRNA gene sequences of strains CIP 102988T, CIP 109878T, CIP 105923T, CIP 103620T and CIP 110030T sequenced in this study are HE573891–HE573896, HE573899–HE573904, HE573907–HE573912 and HE573913–HE573918, respectively.

    • Three supplementary figures are available with the online version of this paper.

    Abbreviations:
    MLSA
    multilocus sequence analysis