Gillisia marina sp. nov., from seashore sand, and emended description of the genus Gillisia

Seong Woon Roh, Myunglip Lee, Hae-Won Lee, Kyung June Yim, Soo Yeon Heo, Kil-Nam Kim, Changmann Yoon, Young-Do Nam, Joon Yong Kim, Dong-Wook Hyun, Jin-Woo Bae, Joon Bum Jeong, Heewan Kang, Daekyung Kim

¹Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea

²Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam 463-746, Republic of Korea

³Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea

⁴School of Marine Biomedical Science, Jeju National University, Jeju 690-756, Republic of Korea

⁵Graduate School of Bio and Information Technology, Hankyong National University, Anseong 456-749, Republic of Korea

Correspondence
Heewan Kang kanghw2{at}hknu.ac.kr Daekyung Kim dkim{at}kbsi.re.kr

International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 10):3640–3645 · https://doi.org/10.1099/ijs.0.048116-0

Abstract

A Gram-staining-negative, strictly aerobic, rod-shaped bacterium, designated CBA3202^T, was isolated from seashore sand on Jeju Island, Republic of Korea. Based on the 16S rRNA gene sequence analysis, strain CBA3202^T was allocated to the genus Gillisia (family Flavobacteriaceae) and was most closely related to the type strain of Gillisia mitskevichiae (99.0 % 16S rRNA gene sequence similarity). Optimal growth occurred at 25 °C and with 3 % NaCl. The only isoprenoid quinone was menaquinone-6 (MK-6), the predominant fatty acids were C_16 : 0, iso-C_15 : 1 G, iso-C_16 : 0 and summed feature 3 (comprising C_16 : 1ω6c and/or C_16 : 1ω7c), and the DNA G+C content was 34.9 mol%. The polar lipids were phosphatidylethanolamine, two unidentified aminolipids and several unidentified polar lipids. Based on phylogenetic inference and phenotypic data, we conclude that strain CBA3202^T represents a novel species of the genus Gillisia, for which the name Gillisia marina sp. nov. is proposed. The type strain is CBA3202^T ( = KACC 16693^T = KCTC 32030^T = JCM 18402^T). An emended description of the genus Gillisia is also provided.