SGM Journals
Actinobacteria

Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., two rapidly growing members of the genus Mycobacterium

Dao-Feng Zhang, Xiu Chen, Xiao-Mei Zhang, Xiao-Yang Zhi, Ji-Cheng Yao, Yi Jiang, Zhi Xiong, Wen-jun Li

  • 1Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China
  • 2Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China
  • 3Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, PR China
  • Correspondence
    Wen-jun Li wjli{at}ynu.edu.cn Yi Jiang jiangyi{at}ynu.edu.cn

    • International Journal of Systematic and Evolutionary Microbiology 2013; 63(Pt 11):4081–4086 · https://doi.org/10.1099/ijs.0.050567-0

    View at publisher PubMed

    Abstract

    Two novel isolates of rapidly growing, Gram-stain-positive, non-chromogenic species of the genus Mycobacterium, strain YIM M13028T from a sediment sample collected from the South China Sea (19° 30.261′ N 111° 0.247′ E) at a depth of 42 m and strain YIM 121001T from a coastal zone sand sample collected in Dubai, United Arab Emirates, were obtained in our laboratory. Their taxonomic positions were determined by a polyphasic approach. Good growth of the two strains was observed at 28 °C and pH 7.0 with 0–2 % NaCl on tryptic soy agar medium. Both strains formed round orange–red colonies, strain YIM M13028T had a rough surface, while YIM 121001T was smooth. Cellular fatty acids, whole-cell protein profiles and TLC analysis of their mycolic acids show significant differences from reference stains. Phenotypic characteristics and multilocus sequence analysis (MLSA) of 16S rRNA gene, hsp65, rpoB and 16S–23S internal transcribed spacer (ITS) sequences indicated that both strains YIM M13028T and YIM 121001T belong to the genus Mycobacterium. DNA–DNA hybridization values revealed a low relatedness (<70 %) of the two isolates with the type strains Mycobacterium neoaurum DSM 44074T and Mycobacterium hodleri DSM 44183T. The low DNA–DNA hybridization values (40.4±3.5 %) between strains YIM M13028T and YIM 121001T and phenotypic distinctiveness indicated that the two strains were representatives of different novel species of the genus Mycobacterium. The names proposed for these novel species are Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., and the type strains are YIM M13028T ( = DSM 45643T = KCTC 19999T) and YIM 121001T ( = DSM 45768T = JCM 18538T), respectively.

    • ↵† These authors contributed equally to this work.

    • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene, hsp65, rpoB and 16S–23S ITS gene sequences of strain YIM M13028T are KC010490, KC010486, KC010488 and KC010492, respectively, and of strain YIM 121001T are KC010491, KC010487, KC010489 and KC010493, respectively. The GenBank/EMBL/DDBJ accession number for the 16S–23S ITS gene sequence of strain DSM 44183T is KC010494.

    • Five supplementary figures and a supplementary table are available with the online version of this paper.

    Abbreviations:
    ITS
    internal transcribed spacer