SGM Journals
Eukaryotic Micro-Organisms

Pseudozyma brasiliensis sp. nov., a xylanolytic, ustilaginomycetous yeast species isolated from an insect pest of sugarcane roots

Juliana Velasco de Castro Oliveira, Thuanny A. Borges, Renato Augusto Corrêa dos Santos, Larissa F. D. Freitas, Carlos Augusto Rosa, Gustavo Henrique Goldman, Diego Mauricio Riaño-Pachón

  • 1Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil
  • 2Departamento de Microbiologia, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, Minas Gerais, Brazil
  • 3Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil
  • Correspondence
    Gustavo Henrique Goldman ggoldman{at}usp.br Diego Mauricio Riaño-Pachón diego.riano{at}bioetanol.org.br

    • International Journal of Systematic and Evolutionary Microbiology 2014; 64(Pt 6):2159–2168 · https://doi.org/10.1099/ijs.0.060103-0

    View at publisher PubMed

    Abstract

    A novel ustilaginomycetous yeast isolated from the intestinal tract of an insect pest of sugarcane roots in Ribeirão Preto, São Paulo State, Brazil, represents a novel species of the genus Pseudozyma based on molecular analyses of the D1/D2 rDNA large subunit and the internal transcribed spacer (ITS1+ITS2) regions. The name Pseudozyma brasiliensis sp. nov. is proposed for this species, with GHG001T ( = CBS 13268T = UFMG-CM-Y307T) as the type strain. P. brasiliensis sp. nov. is a sister species of Pseudozyma vetiver, originally isolated from leaves of vetiver grass and sugarcane in Thailand. P. brasiliensis sp. nov. is able to grow well with xylan as the sole carbon source and produces high levels of an endo-1,4-xylanase that has a higher specific activity in comparison with other eukaryotic xylanases. This enzyme has a variety of industrial applications, indicating the great biotechnological potential of P. brasiliensis.

    • The GenBank/EMBL/DDBJ accession number for the whole-genome shotgun sequence of strain GHG001T is AWXO00000000. The accession number for the ITS1+ITS2 and LSU rDNA D1/D2 regions of strain GHG001T is KF737866.

    • A supplementary taxonomic key is available with the online version of this paper.

    Abbreviations:
    ITS
    internal transcribed spacer
    LSU
    large subunit
    XOS
    xylo-oligosaccharide