Abstract
A species diversity study of lactic acid bacteria occurring in traditional Vietnamese nem chua yielded an isolate, LMG 26767T, that could not be assigned to a validly named species. The isolate was initially investigated by 16S rRNA gene sequence analysis, which revealed that it belonged to the genus Lactobacillus, with Lactobacillus manihotivorans and Lactobacillus camelliae as the closest relatives (98.9% and 96.9% gene sequence similarity towards the type strains, respectively). Comparative (GTG)5-PCR genomic fingerprinting confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridization experiments, DNA G+C content determination, sequence analysis of the phenylalanyl-tRNA synthase (pheS) gene, and physiological and biochemical characterization demonstrated that strain LMG 26767T (= CCUG 62266T) represents a novel species, for which the name Lactobacillus porcinae sp. nov. is proposed. Biochemically, Lb. porcinae can be distinguished from Lb. manihotivorans and Lb. camelliae by its carbohydrate fermentation profile, absence of growth at 45°C, and production of D- and L- lactate as end products of glucose metabolism.