Abstract
We compared the BCC genomovar distribution in CF patients from the Czech Republic and CF patients from the Slovak Republic. BCC isolates from 61 Czech CF patients attending the Prague CF centre and from 24 Slovak CF patients attending three different Slovak CF centres were collected during the year 2001. The BCC was recovered in sputum cultures and verified by means of a nested-PCR assay (Drevinek et al., 2002). The genomovar status of BCC was then determined using a set of eight recA gene sequence-specific PCRs distinguishing all the genomovars with the exception of B. anthina and B. pyrrocinia.
The BCC genomovar distribution in the Czech and Slovak CF populations is shown in Table 1. Whereas genomovar III-A was predominant in the Czech CF community (90 %), in Slovakia the most frequently identified genomovar was B. stabilis (54 %) (P of the difference < 10-4). This result is in marked contrast to what might be expected in two neighbouring populations which had formed the Czechoslovak federation until the end of 1992.