Serotype profile and molecular characterization of antimicrobial resistance in non-typhoidal Salmonella isolated from gastroenteritis cases over nine years

Neelam Taneja, Suma B. Appannanavar, Ajay Kumar, Garima Varma, Yashwant Kumar, Balvinder Mohan, Meera Sharma

¹Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

²National Salmonella and Escherichia Serotyping Centre, Central Research Institute, Kasauli, India

Correspondence
Neelam Taneja drneelampgi65{at}yahoo.com

Journal of Medical Microbiology 2014; 63(Pt 1):66–73 · https://doi.org/10.1099/jmm.0.061416-0

Abstract

Resistance to third-generation cephalosporins in non-typhoidal Salmonella (NTS) is emerging worldwide. We report the occurrence of extended-spectrum beta-lactamase (ESBL) phenotypes in 53.4 % of NTS isolated over a period of nine years from gastroenteritis cases. ESBL and AmpC co-production was observed in 21 % of the isolates. Occurrence of bla_CTX-M-15 and bla_CMY-2 resistance genes was observed in 11.6 % and 37 % of the isolates respectively. Overall, Salmonella enterica serovar Senftenberg was the predominant serovar carrying bla_CTX-M-15 and bla_CMY-2 resistance genes. We report for the first time from India, one isolate each of S. enterica serovar Thompson, S. enterica serovar Infantis and S. enterica serovar Newport, carrying the bla_CTX-M-15 gene. We also report for the first time from India, a case of gastroenteritis due to S. enterica serovar Thompson.