Research Article

Cloning of the Aconitase Gene (acn) of Escherichia coli K12

Journal of General Microbiology 1986; 132(6):1763 · https://doi.org/10.1099/00221287-132-6-1763

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Abstract

Summary: Lambda phages containing the aconitase gene (acn) of Escherichia coli K12 have been isolated by hybridization with an M 13 probe containing part of the aconitase gene (citB) of Bacillus subtilis. Aconitase specific activities are amplified 5- to 18-fold in thermally induced λacn lysogens and threefold in a strain transformed with a plasmid derivative (pGS181).