Abstract
One hundred and fifty-eight strains of vibrio, predominantly Vibrio cholerae, were tested for their nutritional requirements. Half of the strains were able to grow on a simple inorganic medium with ammonium ions as the sole source of nitrogen; the other strains required purines in addition. The simplest purine which produced adequate growth was hypoxanthine. The purine-requiring strains were able to grow on inorganic media containing human, rabbit or goat serum, but not on the serum from mouse, rat, guinea pig or horse.