Abstract
SUMMARY: Sodium chloride decreased the maximum specific growth rate of Saccharomyces cerevisiae. Chemostat experiments showed this to be largely due to an increased requirement for energy-yielding substrate, apparently linked to maintenance and leading to a decrease in the yield. The increased maintenance requirement is probably concerned with maintaining an intracellular Na+ concentration ten times lower than the extracellular concentration. NaCl caused much higher concentrations of glucose to be required to maintain any particular glucose-uptake rate; it also increased the production of glycerol.