Abstract
In KB cells infected with frog virus 3 at 37°, a non-permissive temperature for frog virus 3, there was a marked inhibition of host-cell RNA and DNA synthesis shortly after infection. The inhibition, which was as effective with gamma-irradiated or heat-inactivated virus as with live frog virus 3 was dependent upon the multiplicity of infection with frog virus 3. The effect of frog virus 3 could not be abolished by chemical inhibitors of protein and RNA synthesis. These findings suggested that a component of the virus particle was responsible for the inhibition of host-cell RNA and DNA synthesis.