Abstract
The reproduction of a mutant of A2/SINGAPORE/57, selected after repeated passages in monkey kidney cells, and the synthesis of its subviral components were followed in two systems: in monkey kidney cells, in which new infectious virus is formed, and in human diploid cells, in which the growth cycle of this virus is abortive. The formation of subviral antigens was investigated by both immunofluorescence and complement-fixation tests. No marked differences were found between the kinetics of synthesis of the V and S antigens and their total yields in both systems. Migration of the S antigen from the nuclei was observed in A2/SINGAPORE virus infected by human diploid cells.