Abstract
Dose-response studies were performed in relation to the rate of uptake of [3H]-2-deoxyglucose by murine sarcoma virus-infected murine cells. A linear relationship was observed between the virus dilution used for infection and the rate of dgl uptake by the infected cells. The extrapolated dilution factor (reciprocal of dilution) at which no difference was observed between infected and uninfected cells was directly proportional to the activity estimated by focus assay of MSV. Such end-point dilution factors can be reproducibly and objectively obtained 2 to 4 days after infection.