RNA Synthesis in HeLa Cells infected with Frog Virus 3

The cytoplasmic deoxyvirus frog virus 3 (FV3) can replicate in HeLa cells at a permissive temperature (26 °C). The effect of virus infection on the host-cell RNA metabolism was studied at both permissive and non-permissive (37 °C) temperatures. The inhibition of precursor ribosomal RNA synthesis was shown to take place soon after infection at both temperatures. However, the inhibition of synthesis of heterogeneous nuclear RNA occurs only at the non-permissive temperatures.