Modifications of Cellular RNA-polymerase II after Infection with Frog Virus 3

RNA-polymerase II extracted from FV3-infected and uninfected BHK cells were compared by measuring their abilities to bind [³H]-amanitin and ribonucleoside triphosphates. Binding sites for [³H]-amanitin and the dissociation constant of the complex between [³H]-amanitin and RNA-polymerase II were significantly modified following FV3 infection. The apparent K_m's for ribonucleoside triphosphates remained unchanged.