Research Article

The Fate of Protein Subunits of Parainfluenza (Sendai) Virus after Adsorption to NIL8 Hamster Embryo Cells

Journal of General Virology 1975; 29(2):179 · https://doi.org/10.1099/0022-1317-29-2-179

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Abstract

Adsorption of u.v.-inactivated Sendai virus on to NIL8 hamster cells causes fusion of the cells into polykaryocytes within 2 h. Infected cells were incubated at 37 °C for periods of 10 min to 8 h and their surface proteins iodinated with [125I] catalysed by peroxidase. Structural components of the viral envelope, such as haemagglutinin-neuraminidase (HN) and probably also the fusion protein (F) were detected in the cell membrane for periods up to 4 h post infection.

* Permanent address: Israel Institute for Biological Research, Ness Ziona, Israel.