Research Article

Electron Microscopy of Tobacco Mosaic Virus Prepared with the Aid of Negative Staining-Carbon Film Techniques

Journal of General Virology 1976; 31(2):265 · https://doi.org/10.1099/0022-1317-31-2-265

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Abstract

The negative staining-carbon technique has been applied to suspensions of freshly prepared type strain tobacco mosaic virus particles in high concentrations. Electron microscope images show that paracrystalline arrays of the virus were formed, in which large areas of rods could be viewed along their long axes and in parallel arrays in horizontal positions. High-resolution micrographs showed the protein structure units in rods photographed in both vertical and horizontal orientations.