Research Article

Aminoacylation of Encephalomyocarditis Virus RNA

Journal of General Virology 1977; 34(1):177 · https://doi.org/10.1099/0022-1317-34-1-177

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Abstract

RNA extracted from purified encephalomyocarditis (EMC) virus (EMC-RNA) can be aminoacylated with synthetase preparations from Escherichia coli, beef and rabbit liver. The extent of aminoacylation is between 0·024 and 0·080 moles per mole EMC-RNA and occurs only with serine. Neither removal of possible low mol. wt. contaminants with 3 M-sodium acetate nor periodate oxidation of the virus RNA affects its aminoacylation capacity.