Abstract
Virus-specific antigens in cells infected with herpes simplex virus were separated on a preparative scale by two-stage polyacrylamide-gel electrophoresis. In the first stage, termed electrofiltration, the extract was electrophoresed through 3.5% gel to remove larger aggregates. Antigens in the resulting filtrate were then separated in the second stage by electrophoresis through 7% gel. The protein was recovered by sectioning the gel followed by overnight elution. Recoveries of about 70% were obtained from a 12 to 15 mg. load. Immunodiffusion and analytical electrophoresis both confirmed that separation had been achieved.