Research Article

Effect of Temperature on the Order of Electrophoretic Migration of Influenza Virus Neuraminidase and Nucleoprotein Genes in Acrylamide Gels Lacking Denaturing Agents

Journal of General Virology 1978; 40(1):229 · https://doi.org/10.1099/0022-1317-40-1-229

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Abstract

When subjected to electrophoresis at 33 °C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 °C, however, this order of migration is reversed.