Research Article

Solid Phase Indirect Radioimmunoassays for Rapid Diagnosis of Sindbis Virus Antigen

Journal of General Virology 1978; 41(3):641 · https://doi.org/10.1099/0022-1317-41-3-641

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Abstract

Indirect radioimmunoassays have been developed for the rapid detection of Sindbis virus. Dilutions of Sindbis virus from tissue culture fluids have been immobilized and allowed to react with rabbit anti-Sindbis virus antibodies. The bound antibodies were assayed either by 125I-labelled anti-rabbit IgG-antibodies or alternatively by addition of human complement and 125I-labelled anti-human C1q antibodies or 125I-labelled protein A.