Abstract
Infection of turnip protoplasts with cauliflower mosaic virus (CaMV) was investigated using an immunofluorescent technique. Up to 80% of protoplasts were infected when they were inoculated with 10 µg/ml CaMV, together with 1 µg/ml poly-L-ornithine (PLO) in 20 mM-citrate buffer, pH 5.0. Infection was first detected by fluorescent antibody staining 24 h after inoculation and the CaMV inclusion bodies were observed by electron microscopy of the cytoplasm 72 h after inoculation.