Research Article

Plaque Formation and Purification of BK Virus in Cultured Human Urinary Cells

Journal of General Virology 1980; 50(1):185 · https://doi.org/10.1099/0022-1317-50-1-185

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Abstract

Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.