Abstract
Abteilung für Virologie, Bernhard-Nocht-Institut für Schiffs- und Tropenkrankheiten, Bernhard-Nocht-Str. 74, 2000 Hamburg, 4, West Germany
1 Abteilung für Medizinische Virologie, Hygiene-Institut der Universität, Tübingen, West Germany
We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.